In vitro replication of recombinant plasmids carrying chromosomal segments of Xenopus laevis.
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چکیده
منابع مشابه
In vitro replication of recombinant plasmids carrying chromosomal segments of Xenopus laevis.
Recombinant plasmids carrying a segment of Xenopus laevis chromosomal DNA were constructed with plasmid pBR322 as the vector. A recombinant plasmid pXY65 carrying a 3.2-kilobase BamHI segment of the chromosome of X. laevis has been found to contain a repetitive sequence dispersed throughout the X. laevis chromosomes. This plasmid initiated replication in vitro when the supercoiled circular mole...
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DNA fibre autoradiography has been used to study the effects of temperature on the replication of chromosomal DNA of Xenopus laevis cells in tissue culture at 18, 23 and 28 degrees C. Pulse/stepdown labelling shows that the DNA replicates bidirectionally. Origin-to-origin distances (initiation intervals) vary, but the range of and the mean initiation intervals at all three temperatures are much...
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Details are presented of the in vitro synthesis of double-stranded DNA complementary to purified Xenopus globin messenger RNA, using a combination of reverse transcriptase, fragment 'A' of E. coli DNA polymerase 1 and S1 endonuclease. After selection of duplex DNA molecules approaching the length of Xenopus globin messenger RNA by sedimentation of the DNA through neutral sucrose gradients, the ...
متن کاملChromosome replication in early development of Xenopus laevis.
Eggs of Xenopus laevis contain exceptionally large amounts of materials involved in chromosome replication. This maternal stockpile allows an embryo to produce about 80 000 cells in less than 24 h. The adaptations which achieve this involve the mechanisms of both DNA replication and chromatin assembly.
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At the midblastula transition (MBT) during Xenopus laevis development, zygotic transcription begins [1], and the rapid, early cleavage cycles are replaced by cell-division cycles that lengthen and acquire G (gap) phases [2] and checkpoints [3-5]. This cell-cycle remodeling may result from either a loss of maternal products, the transcription of zygotic genes, or the replacement of maternal prot...
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ژورنال
عنوان ژورنال: Proceedings of the National Academy of Sciences
سال: 1982
ISSN: 0027-8424,1091-6490
DOI: 10.1073/pnas.79.12.3697